Figure 6

ADSC conditioned medium-dependent signaling pathways targeting increase of HL-1 cardiomyocyte proliferation. Representative examples of western blot analyses of phosphorylated STAT3 and phosphorylated Erk1/2 in A, B, C) rnCM stimulated with the conditioned medium of ADSC and IL-1β primed ADSC from normoxic and under hypoxic cultures. The stimulation of rnCM with the serum free conditioned medium of ADSC resulted in activation of STAT3 and Erk1/2. D, E, F) in HL-1 cardiomyocytes in the presence of STAT3 phosphorylation inhibitor (Stattic) G, H, I) MEK inhibitor (U0126) and J, K, L) both inhibitors (Stattic + U0126). Inhibition of STAT3 phosphorylation resulted in increased of Erk1/2 phosphorylation. Non-phosphorylated STAT3 and Erk1/2 levels did not change and indicate equivalent protein controls in all samples (n = 3).