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Figure 4 | Journal of Translational Medicine

Figure 4

From: Adipose stromal cells primed with hypoxia and inflammation enhance cardiomyocyte proliferation rate in vitro through STAT3 and Erk1/2

Figure 4

Conditioned medium of ADSC augments the cell cycle progression gene expression profile of HL-1 cardiomyocytes. Quantitative RT-PCR analysis of gene transcript levels of cyclin D1, cyclin D2 and c-Myc normalized to Beta-2 microglobulin expression. A) Gene transcript level of cyclin D1 and cyclin D2 did not change in HL-1 cardiomyocytes upon stimulation with IL-6 or ADSC conditioned medium in normoxia (p < 0.05). B) Gene expression of cyclin D2 showed a significant increase upon stimulation with IL-1β primed conditioned medium of ADSC under hypoxia (p < 0.05). A) Gene transcript levels of c-Myc were significantly upregulated upon stimulation with IL-6 or conditioned medium of ADSC and IL-1β primed ADSC conditioned medium under normoxia (p < 0.05). B) Under hypoxia gene transcript levels of c-myc in HL-1 cells showed significant upregulation upon stimulation with conditioned medium of ADSC and IL-1β primed conditioned medium of ADSC (p < 0.05). A, B) Gene transcript levels of apoptotic marker Bclx remained unchanged in HL-1 cardiomyocytes upon stimulation with IL-6 or conditioned medium of ADSC (p < 0.05). Graphs represent triplicates (with SEM) data from n = 3 independent experiments, normalized to serum-free HL-1 cardiomyocytes.

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