Figure 1

ADSC promote cardiomyocyte proliferation rate in direct co-culture. A) Rat neonatal cardiomyocytes and C) HL-1 cardiomyocytes were co-cultured with mitomycin-C treated ADSC in a 1:1 and 1:3 ratio or none, for 24h. ADSC enhanced rnCM in 1:1 ratio and HL-1 cells proliferation in both ratios (1 F, H). Representative micrographs of BrdUrd fluorescent staining (A, C) and phase contrast (D) are shown. Proliferation was assessed by immunofluorescent detection of 6h incorporated BrdUrd and quantification with Tissue Gnostics Tissue FAXS equipment. B) CM-DiI (red) labeled rnCM were co-cultured in absence, 1:1 and 1:3 ratio of CFDA SE (green) labeled ADSC. Increase densities of rnCM were detected in 1:1 ratio co-culture with ADSC (p < 0.05 1) G) dTomato lentivirally-tagged HL-1 cardiomyocytes were co-cultured in absence, 1:1, 1:2, 1:3 or 1:4 excess of eGFP lentivirally-tagged ADSC. Increased densities of dTomato HL-1 cardiomyocytes were detected after co-culture with eGFP positive ADSC in all ratios (p < 0.05, 1 I). E) Representative immunofluorescent micrographs of dTomato HL-1 cells in co-culture with eGFP ADSC are shown. Cardiomyocyte density was assessed by immunofluorescence detection of dTomato positive HL-1 cells and quantification with Tissue Gnostics Tissue FAXS equipment. Graphs represent triplicates (with SEM) data from n = 3 independent experiments from 3 donors.