Figure 3

Cytotoxic capacity of TIL expanded with IL-15, IL-21 or no cytokine producing aAPC. Percentage of granzyme B- or perforin-expressing CD8⁺ T cells was determined by flow cytometry. A) Dot plots with percentages of granzyme B⁺ cells (top panel) and perforin⁺ cells (bottom panel) within the CD8⁺ T cell population are shown for a representative patient. B) Percentage of granzyme B⁺ cells (left) and perforin⁺ cells (right) within the CD8⁺ T cell population are shown for 12 patients. Differences were compared using repeated measures ANOVA’s with a Tukey post test when data followed Gaussian distribution and by using a Friedman test with a Dunn’s post test when data did not pass the normality test. Differences were considered significant when p < 0.05, as indicated with an asterisk (* p < 0.05, ** p < 0.01), *** p < 0.001).