Growth inhibition and apoptosis induced by hirsutanol A in SW620 and MDA-MB-231 cancer cells. Cells were treated with different concentrations of Hirsutnol A for 72 h, 48 h, 24 h respectively. The inhibitory rate was detemined by MTT assay (A). Cells were treated with various concentrations of hirsutanol A for 72 h, AnnexinV/PI analysis were used to detect the cell apoptosis (B). Proteins were harvested from the SW620 and MDA-MB-231 cells after treatment with indicated concentrations of hirsutanol A for 48 h. Cleavage fraction of PARP and Caspase-3 were detected by immunoblotting assay (C). Results are presented as means ± SD from 3 independent experiments.