E2A suppresses CRC cells invasion and migration. A, Effect of shE2A on E2A expression. Left panel: Immunoblot showed E2A was reduced in shE2A treated SW480 cells compared to blank and shNC treated ones. GAPDH was used as loading control. Right panel: Relative E2A mRNA expression of shE2A-, shNC-treated and Blank SW480 cells. B, Transfection of plasmids pEZ-M29-E12 and pEZ-M29-E47 increased E2A mRNA and proteins expression. C, Downregualtion of E2A increased invasion and migration abilities of SW480 cells. D, Forced ectopic E12 or E47 expression suppressed invasion and migration abilities of SW480/shE2A cells. Representative photos of stained cells are shown with the original magnification of 100×. Scale bars: 100 μm Data represents the means ± SD from 3 independent experiments. *, P < 0.05; **, P < 0.01.