Table 1 Primer sequences for housekeeping, cytokine and endothelial marker quantification by real time RT-PCR

Accession #

E (%)

β-actin

NM_001101

FW -GCG AGA AGA TGA CCC AGA TC-

RW -GGA TAG CAC AGC CTG GAT AG-

100

VEGF-A

NM_001025366

FW -ATG AGG ACA CCG GCT CTG ACC A-

RW -AGG CTC CTG AAT CTT CCA GGC A-

94.5

Angio-1

NM_001146

FW -GAG GCA CGG AAG GAG TGT GCT G-

RW -CGG CGC TGA TTG CTG CAC CCT A-

92.0

Angio-2

NM_001147

FW -AAA AGC TGA CAC AGC CCT CCC A-

RW -ACT GCT GTG TTC TCT CCA GGC A-

86.0

CXCL12

NM_000609

FW -ATG CCC ATG CCG ATT CTT CG-

RW -GCC GGG CTA CAA TCT GAA GG-

100

VE-cadherin

NM_001795

FW -GGT CCC TGA ACG CCC TGG TAA-

RW -GGA GTG GAG TAT GGA GTT GGA GCA-

95.5

von Willebrand Factor (vWF)

NM_000552

FW -GCC TGC TTC TGC GAC ACC ATT G-

RW -GCC ACT CAC ACT CAT ACC CGT TCT-

96.2

Flt1 (VEGF-R1)

NM_002019

FW -CAC GCA GGA CCA GTT TGA TTG AG-

RW -GCT AGA GGA CTC CCG AGA TGT TG-

96.7

Flk-1/KDR

(VEGF-R2)

NM_002253

FW -GCA GGG GAC AGA GGG ACT TG-

RW -GAG GCC ATC GCT GCA CTC A

95.3