Figure 7

In vitro angiogenic potential of PT45-CM treated CD34⁺cells. CD34⁺ cells were grown on Matrigel for 24 h, after being cultured for 19 days under conditions supporting their differentiation toward myeloid lineage cells, in the absence (A) or presence (B) of PT45-CM, pre-treated or not with a neutralizing anti-Angio-1 antibody (C) and with a neutralizing anti-VEGF-A (D) antibody. HUVEC (E) were used as positive control. The figure is representative of three experiments done in duplicate; the results are presented as median (range) values of the angiogenic numerical score, derived from the formula reported in the Materials and Methods section.