Figure 5

Levels of VE-cadherin, vWF, VEGF-R1 and VEGF-R2 mRNA in CD34⁺cultured with or without PT45-CM. Cells were cultured for 19 days under conditions supporting their differentiation toward myeloid lineage cells, in the presence or absence of PT45-CM. Results were assessed by real time RT-PCR, and normalized to β-actin mRNA levels. Normalized gene expression values relative to that of control CD34⁺ cells (cultured in the absence of PT45-CM, arbitrarily set to 1) are shown.