Figure 4

Fluorescence staining of cultured CD34⁺cells with Dil-AcLDL and Ulex europeus lectin under an LSCM. Circulating CD34⁺ cells were cultured for 19 days in the presence of GM-CSF, IL-3 and SCF, either without or with PT45-CM. The laser band of excitation was 488/543 nm; the emission was set with a 560 nm long-pass filter for Dil-AcLDL (red) and a 505–530 nm band pass for FITC conjugated Ulex europeus lectin (green). Photographs are from one representative experiment. The lens used was 20×/0.5 (0.849 × 0.849 mm² image dimension). The fluorescent images were processed using an LSCM 510 Image Examiner Program from Zeiss: the yellow merged image represents the result of double-fluorescence labeling.