Skip to main content
Figure 2 | Journal of Translational Medicine

Figure 2

From: Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors

Figure 2

Chemo-attraction of CD34+cells by PT45-CM and H6c7-CM. PB CD34+ cells and HUVEC were seeded into the upper compartment of a chemotaxis chamber. PT45- or H6c7-CM (30%) was placed in the lower compartment, as such or pre-treated with neutralizing anti-VEGF-A and anti-Angio-1 antibodies. In selected experiments, CD34+ cells were pre-treated with a neutralizing anti-VEGF-R1 antibody before exposure to PT45- or to H6c7-CM. After 24 hr incubation, cells in the upper compartment were removed, and the number of CD34+ or HUVEC cells that had migrated from the upper compartment through the filter into the lower compartment was determined, by counting the cells in three random high-power fields. (A) Representative images of HUVEC and CD34+ cells migrating in response to medium alone (control), to PT45-CM or to H6c7-CM, in a transwell migration assay. (B) Number of cells migrating in a transwell migration assay, in the absence or presence of PT45-CM or H6c7-CM. Results are mean values ± SE of six separate series of studies for PT45-CM and of three series for H6c7-CM. (C) Inhibitory effect of a neutralizing anti-VEGF-R1 antibody on CD34+ cell chemotaxis; values are expressed as percentage inhibition of CD34+ cell migration in the presence of PT45-CM/H6c7-CM versus medium alone (control). Data shown are means ± SE from three separate series.

Back to article page