Gene silencing of TPX2 expression in colon cancer cells suppresses MMP-2 activity. The expression or enzyme activity of TPX2 on SW620 cells treated with shRNA-TPX2, shRNA (control), or Oligofectamine alone (mock) was analyzed by RT-PCR (A), western blot (B), and zymography assay (C). (D) Immunofluorescence microscopic analyses of MMP-2. SW620 cells were infected with shRNA-TPX2 or control cells (control shRNA and mock). The cells were then immunostained for TPX2 (green) and MMP-2 (red), their nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue).