TPX2 expression and pathologic features in colon cancer specimens. (A) Immunohistochemical staining of TPX2 in TMA. TPX2 was localized within the nuclei. Elevated TPX2 expression in the tumor cells of colon cancer tissue (A2, A3, and A4) compared to adjacent normal mucosa with negative staining (A1) (original magnification: 400X for the inserts, 100X for all). (B) The metastasis-free survival (MFS) and overall survival (OS) rates were estimated by the Kaplan–Meier method. Both the MFS rate and OS rate of patients with TPX2 positive primary tumor were significantly lower than that of patients with TPX2 negative primary tumor (log-rank test, P < 0.001).