Protein expression during post-differentiation. A) Phase contrast images in post-differentiation phase (72 and 96 hours) showed hypertrophy in neo-formed myotubes treated with RSV, in respect with control. Scale bar 200 μm. B) Western Blot analysis shows how RSV treatments during post-differentiation phase cause a significant increase in MyHC protein content. Key structural N-Cadherin protein expression is significantly higher than DM. RSV treatments seem to induct a significant rise in AMPK protein content in respect to DM. Significance: * p ≤ 0.05; ** p ≤ 0.01. Representative immunoblots of analyzed proteins are shown. C) Immunofluorescence during post-differentiation phase (8 h treatment), with antibodies anti N-Cadherin and p120 Catenin, shows the main increase in size of neo-formed myotubes treated with RSV in respect to DM condition. Scale bar 200 μm.