Figure 5

RSV role on differentiation phases and cytoskeletal protein. A) RSV treatment seems to cause an anticipate decrease of early MRF Myf-5 in respect to DM control. Myogenin protein content confirms how both 0.1 and 25 μM RSV treatments could anticipate the MRFs expression in respect to DM. RSV reveals an imperative action on protein content rise of key structural proteins like N-Cadherin, Alpha-Sarcomeric Actinin and p120. MyHC blot shows how RSV causes an important increase in MyHC protein levels corroborate to DM. Significance: *p ≤ 0.05; **p ≤ 0.01. Representative immunoblots of analyzed proteins are shown. B, C) MyHC and Alpha Actinin Sarcomeric immunofluorescence images collected at 48 h of differentiation show a major density of cells positive for these two structural proteins in RSV conditions in respect to control conditions. Scale bar 200 μm.