RSV action on cell cycle regulation in proliferation phase. A) Real-Time-PCR during proliferation phase (24 h), proves an actual decrease in Cyclins expression levels after treatment with RSV, in a similar way to DM condition, in respect to GM time 0 condition. Significance: * p ≤ 0.05; ** p ≤ 0.01. B) p53 Western Blot analysis, during proliferation phase, shows how RSV treatment does not modify p53 protein amount in respect to GM control condition. p21 Western Blot analysis reveals a significant decrease in protein content in both 0.1 and 25 μM RSV-treated cells, following DM trend, in respect to growth control GM. Significance: * p ≤ 0.05; ** p ≤ 0.01. Representative immunoblots of analyzed proteins are shown. C) Phase contrast images collected at 24 and 72 h of proliferative phase show morphological changes mentioned in Figure 2. Scale bar 200 μm.