Differentiation of GI pathologies from controls by miRNA biomarker pairs. The concentrations of miRNAs in plasma samples of patients with four GI pathologies, and healthy donors were measured by RT-qPCR and the ratios of levels of various miRNAs were calculated as 2-ΔCq x 100. A-E – box-plots. Here and in other figures with box and whisker plots the results are presented in the log10 scale. The upper and lower limits of the boxes and the lines inside the boxes indicate the 75th and 25th percentiles and the median, respectively. The upper and lower horizontal bars denote the 90th and 10th percentiles, respectively. The points indicate assay values located outside of 80% data. A-D – individual pathologies (10 patients in each group) against controls (30 subjects); E – combined GI pathologies (40 patients total) against controls (30 subjects). F –ROC curves of differentiation between patients with four GI pathologies and controls obtained with different biomarker pairs. The areas under the ROC curves (AUC) are reported. Sensitivity, specificity and accuracy for each miRNA pair are calculated for the “cutoff” point (indicated as a dot on each plot) – the value of the ratio where the accuracy of predictions is the highest (see Methods, ref. 14).