Figure 4

The alternation of the Wnt/beta-catenin signal pathway. The U251 and U87 cells were transfected with either the miR-92b mimics (100 nM), the control oligonucleotide (100 nM) or the miR-92b inhibitor (100 nM). (A) The beta-catenin protein levels were assessed 48 h after the transfection. β-actin was used as the loading control. The Western blots are representative of three independent experiments. Scanning densitometry analysis of the blots was used to quantify the data. (B) Bcl2, c-myc, c-Jun (total and phosphorylated), Caspase-3 and Bax protein levels were assessed 48 h after transfection with either the miR-92b mimics, the control oligonucleotide and the miR-92b inhibitor. β-actin was used as the loading control. Scanning densitometry analysis of the blots was used to quantify the data. The data were expressed as the mean ± SD and were representative of an average of three independent experiments. Significant differences from the control were indicated by **p <0.01 and ***p <0.001.