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Figure 2 | Journal of Translational Medicine

Figure 2

From: MiR-92b inhibitor promoted glioma cell apoptosis via targeting DKK3 and blocking the Wnt/beta-catenin signaling pathway

Figure 2

The effects of miR-92b on proliferation and apoptosis in U251 and U87 cells. (A) Realtime PCR analysis of miR-92b expression in human astrocyte cell line (HA) and glioma cell lines (including U251, U87, SHG44 and A172). The average miR-92b expression was normalized by U6 expression. Each bar represents the mean of three independent experiments. ***p<0.001. (B) After transfection, U251 and U87 cells were counted and seeded separately in 12-well plates. The colonies were counted on the sixth day after seeding and the rate of colony formation was calculated. All the data were representative of three independent experiments. (C) The rates of cell growth were measured using an MTT assay. All the cells were seeded into 96-well culture plates. The absorbance at 492 nm was measured. MiR-92b mimics increased cell viability (**p<0.01). Conversely, miR-92b inhibitors showed a significant decrease in cell viability (***p<0.001). All the data were presented as the mean ± SD of a representative average of three independent experiments. (D) Three days after the transfection, the apoptosis was assessed using Annexin V-FITC analysis. Compared to the control oligonucleotide group, a significant increase in cellular apoptosis was inducted by miR-92b inhibitors, whereas the miR-92b mimics group showed a decrease in cellular apoptosis. The bar chart represents our repeating results. All data were presented as means ± SD and as representative of an average of three measurements.

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