Figure 5

PTTG1 was identified as a target of miR-655 in EC9706 and KYSE150 cells. (a) The putative miR-655 binding sequences for the PTTG1 3’ UTR. (b) Western blot analysis of PTTG1 expression in transfected cells. β-actin was used as a reference. Blank, non-transfected cells; NC, cells transfected with scrambled miR-655 negative control; miR-655, cells transfected with miR-655 mimics. (c) Luciferase activity determined 48 h after transfection. NC, scrambled-miRNA; miR-655, miR-655 mimics; Wt-PTTG1, pGL3-PTTG1-wt; Mut-PTTG1, pGL3-PTTG1-mut. ^*P < 0.05 compared to the control group.