Humoral immune response after vaccination with Hsp70-Mage-a1 fusion protein. Purified GST, Hsp70, Mage-a1, Mage-a1-Hsp70 fusion protein or a combination of Mage-a1 + Hsp70, were diluted to a concentration of 200 pmol. Mice were immunized sub-cutaneously with a PBS control or 0.2 ml of each protein, followed by a booster injection after one week. Serum samples were obtained two weeks after the booster. The levels of anti-Mage-a1 antibody were examined by ELISA. The means ± SE of the absorbance (A540 nm) of three separate experiments are presented at 1:50 dilution. Statistical analysis by one-way ANOVA showed that mice vaccinated with Mage-a1-Hsp70 had significantly higher titers of anti Mage-a1 antibody than Mage-a1 or Mage-a1 + Hsp70 (* p < 0.001).