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Table 2 Effect of L-NAME and apocynin on the efficacy of and sensitivity to acetylcholine in isolated aortic rings from wild-type control (WT) mice and from vehicle- and sildenafil-treated apolipoprotein E-deficient (apoE−/−) mice

From: Sildenafil restores endothelial function in the apolipoprotein E knockout mouse

Curve parameter

WT

   apoE−/−

 

Vehicle

Sildenafil

Acetylcholine

   

 Rmax (%)

87 ± 3.6

66 ± 9.7*

95 ± 3.1#

 pEC50

7.3 ± 0.1

6.1 ± 0.1**

7.2 ± 0.3##

Acetylcholine + L-NAME

   

 Rmax (%)

11 ± 2.0§§

18 ± 4.4§§

12 ± 2.8§§

 pEC50

8.7 ± 0.5§

8.5 ± 0.2§§

7.3 ± 1.1

Acetylcholine + apocynin

   

 Rmax (%)

84 ± 5.2

101 ± 4.6§§

94 ± 3.7

 pEC50

7.3 ± 0.2

7.3 ± 0.2§§

7.2 ± 0.2

  1. Rmax, maximal response (efficacy). pEC50, the negative logarithm of the concentration required to produce 50% of the Rmax (sensitivity). Values are mean ± SEM of 5–10 mice per group. *p < 0.05 and **p < 0.01 vs. WT and #p < 0.05 and ##p < 0.01 vs. apoE−/− vehicle (one-way ANOVA); §p < 0.05 and §§p < 0.01 vs. respective control without blockade (two-way ANOVA).
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Journal of Translational Medicine

ISSN: 1479-5876

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