Figure 1

Gene expression analysis of BMSCs co-cultured with leukemia cells compared with BMSC mono-cultures shows changes in IL-17 signaling-related genes. (A) Hierarchical clustering analysis of 1540 differentially expressed genes in BMSCs co-cultured in transwells with three leukemia cell lines (TF-1, TF-1α and K562) compared with BMSC mono-cultures (control) using Partek Genomic Suite program (ANOVA test with unadjusted p-value < 0.05). The displayed colors represent the fold changes where shades of red and blue indicate up- and down-regulation respectively. The color key for the sample labels is on the top left. (B) Ingenuity Pathway Analysis (IPA) of the 1540 differentially expressed BMSC genes. Numerical symbols at the right side of each bar indicate the total number of genes composing the pathway. The bars indicate the percentage of up- (red bar) and down-regulated (green bar) genes in each pathway, while the orange line indicates minus-log transformed p-value. The top 10 canonical pathways are shown.