Figure 3
Effects of Cdc42 inhibition by AZA197 on cell proliferation in SW620 colon cancer cells. A Relative density of cancer cells up to 72 h following treatment with 1, 2, 5, and 10 μM compound AZA197 was measured using the WST-1 cell proliferation assay. AZA197 suppresses SW620 colon cancer cell proliferation in a dose-dependent manner. Means of three independent experiments are shown. *, significantly different from control. B Cellular DNA content was analyzed by flow cytometry after staining with propidium iodide. Representative flow cytometry histograms showing SW620 cells treated with 2, 5 or 10 μM AZA197 for 24 h. Control cells received no treatment. AZA197 dose-dependently reduces the number of cells in the S and G2/M phases (M3) and increases the number of cells with sub G0/G1 DNA content (M1) characteristic of apoptosis.