Figure 3

Alpha-SMA (α-SMA) Vimentin (VIM) and Fibronectin (FN) gene expression after Everolimus (EVE). Histograms represent A) α-SMA, B) VIM and C) FN mRNA levels determined by real-time PCR. WT and silenced cell lines were cultured with Everolimus (10, 100, 200 and 500 nM) for 6 h. FGF-2 (10 ng/ml) was used as positive control. Mean ± S.D. (error bars) of three separate experiments performed in triplicate. *p < 0.05 vs WT control cells (CTR); **p < 0.001 versus WT CTR.