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Figure 2 | Journal of Translational Medicine

Figure 2

From: Everolimus-induced epithelial to mesenchymal transition in immortalized human renal proximal tubular epithelial cells: key role of heparanase

Figure 2

Matrix metalloproteinase-9 (MMP9) gene expression and activity after Everolimus (EVE) treatment. (A) Histograms represent MMP9 mRNA expression levels determined by real-time PCR in WT and HPSE silenced HK-2 cells (shHPSE). WT and silenced cell lines were cultured with 10, 100, 200 and 500 nM Everolimus for 6 h. FGF-2 (10 ng/ml), a well-known EMT inducer, was used as positive control. Mean ± S.D. (error bars) of three separate experiments performed in triplicate. *p < 0.05 vs WT control cells (CTR); **p < 0.001 versus WT CTR. (B) Upper, gelatin zymography shows MMP-9 activity bands in the serum-free conditioned media of WT and silenced HK2 cells. WT and silenced cell lines were cultured with 10, 100, 200 and 500 nM Everolimus for 24 h, then conditioned media were harvested. Lower, histogram represents the densitometric analysis of relative MMP-9 activity. Mean ± S.D. (error bars) of three separate experiments performed in triplicate. *p < 0.05 vs WT control cells (CTR); **p < 0.001 versus WT CTR.

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