Effects of SelS on the expression of Cav-1 in ECs stimulated with or without H
. A. The level of Cav-1 gene expression presented as a ratio to GAPDH following real-time PCR analysis. B. The level of Cav-1 protein expression presented as a ratio to β-actin following densitometric analysis of western blots. C. Western blot analysis of Cav-1 expression in HUVECs not stimulated with H2O2. D. Western blot analysis of Cav-1 expression in HUVECs stimulated with 800 μmol/L H2O2. White column: no H2O2 stimulation; shaded column: stimulation with 800 μmol/L H2O2. 1, 2, 3, 4: control group, pc-SelS group, vector control group and siRNA-SelS group, respectively. #P < 0.01 compared with the corresponding control groups not stimulated with H2O2; *P < 0.01 compared with the vector control group; $P < 0.01 compared with the SelS over-expression group. n = 3.