Wound outcome in combat injuries is associated with a unique set of protein biomarkers

Chromy, Brett A; Eldridge, Angela; Forsberg, Jonathan A; Brown, Trevor S; Kirkup, Benjamin C; Jaing, Crystal; Be, Nicholas A; Elster, Eric; Luciw, Paul A

doi:10.1186/1479-5876-11-281

Journal of Translational Medicine

Table 2 Proteins showing differential responses

From: Wound outcome in combat injuries is associated with a unique set of protein biomarkers

Spot No.	IPI No.	Gene Name	Protein Name	Mascot Score	Fold Change (Healed/ Dehisced)	T-test	Th. MW	Th. pI
59	IPI00440484	PER1	Period Circadian protein homolog 1	133	-1.23	0.0414	137667	5.73
95*	IPI00645206	PCDH17	Isoform 1 of Protocadherin 17	131	-1.22	0.0121	127405	5.03
99*	IPI00235407	STK36	Isoform 1 of Serine/threonine protein kinase 3	142	-1.24	0.0018	145728	5.57
102	IPI00185661	USP32	Ubiquitin carboxyl terminal hydrolase 32	125	-1.2	0.0164	145671	6.18
106	IPI00470744	PARG	Isoform 2 of Poly (ADP-ribose) glycohydrolase	113	-1.22	0.0106	103503	5.58
168*	IPI00290328	PTPRJ	Receptor type tyrosine protein phosphotase precursor	130	-1.51	0.0061	152960	5.8
195*	IPI00017601	CP	Ceruloplasmin	101	-1.27	0.0116	122983	5.44
198*	IPI00017601	CP	Ceruloplasmin	121	-1.39	0.0076	122983	5.44
199	IPI00017601	CP	Ceruloplasmin	182	-1.31	0.0095	122983	5.44
200	IPI00017601	CP	Ceruloplasmin	116	-1.33	0.0075	122983	5.44
201	IPI00017601	CP	Ceruloplasmin	112	-1.3	0.0254	122983	5.44
202	IPI00017601	CP	Ceruloplasmin	154	-1.31	0.0130	122983	5.44
329	IPI00011736	PIK3R2	Phosphatidylinositol 3 kinase regulatory subunit 2	88	1.26	0.0259	81837	6.03
330	IPI00783987	C3	Complement C3	115	1.55	0.0003	188569	6.02
333	IPI00011736	PIK3R2	Phosphatidylinositol 3 kinase regulatory subunit 2	111	1.25	0.0046	81837	6.03
337	IPI00783987	C3	Complement C3	88	1.53	0.0104	188569	6.02
341*	IPI00783987	C3	Complement C3	147	1.56	0.0006	188569	6.02
343*	IPI00783987	C3	Complement C3	189	1.62	0.0006	188569	6.02
344	IPI00783987	C3	Complement C3	120	1.69	0.0048	188569	6.02
345	IPI00783987	C3	Complement C3	79	1.59	0.0125	188569	6.02
347	IPI00783987	C3	Complement C3	104	1.52	0.0339	188569	6.02
375*	IPI00607814	XPNPEP1	Xaa-Pro aminopeptidase 1 Isoform 2	121	1.23	0.0415	72746	5.67
389	IPI00157535	EPS8L1	Isoform 4 of Epigrowth factor receptor kinase subunit 8	118	1.23	0.0168	88920	6.49
436	IPI00009089	FRS3	Fibroblast growth factor receptor substrate 3	121	-1.28	0.0198	55169	6.81
582	IPI00301255	IGSF21	Immunoglobulin superfamily member 21	109	-1.23	0.0480	51855	6.48
602	IPI00329555	F7	Isoform A of Coagulation factor VII	178	-1.54	0.0232	53043	6.91
607	IPI00329555	F7	Isoform A of Coagulation factor VII	130	-1.37	0.0462	53043	6.91
787	IPI00021891	FGG	Fibrinogen gamma chain 1	135	1.33	0.0267	51511	5.37
794	IPI00847635	SERPINA3	Isoform 1 of alpha-1-antichymotrypsin	146	1.57	0.0067	47792	5.33
800	IPI00847635	SERPINA3	Isoform 1 of alpha-1-antichymotrypsin	216	1.22	0.0273	47792	5.33
814	IPI00219330	ILF3	Isoform 5 of Interleukin enhancer binding factor 3	252	-1.35	0.0205	74959	8.4
869	IPI00783625	SERPINB5	Isoform 1 of Serpin B5	120	1.27	0.0356	42530	5.72
872*	IPI00847635	SERPINA3	Isoform 1 of alpha-1-antichymotrypsin	111	1.39	0.0456	47792	5.33
876	IPI00847635	SERPINA3	Isoform 1 of alpha-1-antichymotrypsin	283	1.24	0.0424	47792	5.33
976	IPI00004657	HLA-B	HLA class 1 histocompatibility antigen	102	1.27	0.0150	40777	5.57
980	IPI00004657	HLA-B	HLA class 1 histocompatibility antigen	121	1.34	0.0383	40777	5.57
981	IPI00004657	HLA-B	HLA class 1 histocompatibility antigen	101	1.57	0.0008	40777	5.57
1080	IPI00166729	AZGP1	Zinc alpha 2 glycoprotein precursor	147	-1.34	0.0031	34258	5.71
1177	IPI00555812	GC	Vitamin D binding protein precursor	104	-1.28	0.0151	52963	5.4
1186	IPI00641737	HP	Haptoglobin	92	-1.29	0.0374	45205	6.13
1374	IPI00978715	CLU	Clusterin	109	-1.25	0.0106	52495	5.88
1381	IPI00410313	KIR3KL3	Killer cell immunoglobulin like receptor 3DL3	132	1.25	0.0144	45470	7.27
1392	IPI00022391	APCS	Serum Amyloid P component	181	1.48	0.0223	25837	6.1
1487	IPI00386246	AMPH	Amphiphysin I variant CT3, fragment	119	1.24	0.0251	31195	4.23
1796	IPI00007879	SRF	Serum response factor	132	1.21	0.0460	51592	7.83

The 45 identified differential spots found among biological replicates of healed versus dehisced wounds (corresponding to t-test < 0.05 and fold change cutoff > 1.2) were identified via LC-MS/MS and yieled 25 unique protein identifications. The table includes spot number, IPI database number, gene name, protein name, MASCOT identification score, fold change and t-test of expression between healed and dehisced wounds, theoretical molecular weight and pI. *Indicates the 9 proteins selected as markers by discriminate analysis. Spot numbers 332, 336, 548, 763, 978, and 1797 were unable to be confidently identified.

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ISSN: 1479-5876

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