Figure 1

Wnt signaling pathway is activated in TNBC cells. Subcellular localization of β-catenin in TNBC cells treated with or without human recombinant Wnt-3a (200 ng/ml) for 4 hours was examined using confocal microscopy. Immunofluorescence staining of β-catenin (green) showed cytoplasmic localization in MCF-7 cell line, and was both nuclear and cytoplasmic in TNBC cell lines, MDA-MB-231 and BT-549. Treatment with Wnt-3a resulted in increased nuclear localization of β-catenin in MDA-MB-231 and BT-549 cells. Nuclei were counterstained with Hoechst 33342 (blue). Total magnification was 200×, and the images were zoomed in 500%.