Figure 1

Amniochorionic membrane based 3D model: involvement of the amniochorionic membrane scaffold (AMS) by ovarian cancer cell lines compared to histologic involvement of the peritoneum. A. Picture by fluorescence microscopy of Ovcar3_eGFP aggregates after 24 hours 3D culture (upper picture). Early attachement (day 1) of the tumoral aggregates on AMS in fluorescence microscopy (lower picture). B. Early involvement of AMS stained with CD73 APC (red) by Ovcar3_eGFP (green) in confocal microscopy. On this Z-stack reconstruction of a small tumor, we could see the pattern of eGFP-OCC infiltration within the AMS. Amniotic stromal cells CD73+ were located in surface but also deeper within AM, surrounding OCCs. C-F. On the histologic sections stained with hematoxylin & eosin, the area below the mesothelium (star) of a normal peritoneum (C) and amniochorionic membrane (E) was free whereas it was filled with tumoral cells (rhombus) in sections of peritoneal metastasis (D) and involved AMS at day 2 (F).