Figure 3

Insufficient RFA promoted EMT of SMMC7721 cells through Akt and ERK1/2 signaling pathways. (A) The expression of p-Akt, Akt, p-ERK1/2, ERK2 and snail in SMMC7721 and SMMC7721-H cells were assessed by western blot. (B) LY294002 or PD98059 was used to treat HCC cells, and western blot was used to determined the expression of p-Akt, Akt, p-ERK1/2, ERK2, E-cadherin, N-cadherin, and snail. (C-D) LY294002 or PD98059 was used to treat SMMC7721 and SMMC7721-H cells, and migration (C) and invasion (D) of SMMC7721 and SMMC7721-H were evaluated. Error bars represent the SEM of data obtained in three independent experiments. P value <0.05 was considered statistically significant; ***p < 0.001, **p < 0.01, ns, no significance.