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Figure 1 | Journal of Translational Medicine

Figure 1

From: Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathways

Figure 1

Insufficient RFA promoted the proliferation, colony formation, migration and invasion of HCC cells. SMMC7721 and Huh7 cells were treated with insufficient RFA (47°C 5 min, 10 min, 15 min, 20 min and 25 min) gradually. Residual SMMC7721 and Huh7 (named as SMMC7721-H and Huh7-H respectively) cells were collected and used for the next experiments. (A) Proliferation rate of SMMC7721, SMMC7721-H, Huh7 and Huh7-H was evaluated by MTT assay. Error bars represent the SEM of data obtained in five independent experiments. (B) Colony formation abilities of SMMC7721, SMMC7721-H, Huh7 and Huh7-H cells were assessed. Error bars represent the SEM of data obtained in three independent experiments. (C) Migration and invasion of SMMC7721, SMMC7721-H, Huh7 and Huh7-H cells were shown. Error bars represent the SEM of data obtained in three independent experiments. P value <0.05 was considered statistically significant; ***p < 0.001, **p < 0.01.

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