Figure 1From: Insufficient radiofrequency ablation promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells through Akt and ERK signaling pathwaysInsufficient RFA promoted the proliferation, colony formation, migration and invasion of HCC cells. SMMC7721 and Huh7 cells were treated with insufficient RFA (47°C 5 min, 10 min, 15 min, 20 min and 25 min) gradually. Residual SMMC7721 and Huh7 (named as SMMC7721-H and Huh7-H respectively) cells were collected and used for the next experiments. (A) Proliferation rate of SMMC7721, SMMC7721-H, Huh7 and Huh7-H was evaluated by MTT assay. Error bars represent the SEM of data obtained in five independent experiments. (B) Colony formation abilities of SMMC7721, SMMC7721-H, Huh7 and Huh7-H cells were assessed. Error bars represent the SEM of data obtained in three independent experiments. (C) Migration and invasion of SMMC7721, SMMC7721-H, Huh7 and Huh7-H cells were shown. Error bars represent the SEM of data obtained in three independent experiments. P value <0.05 was considered statistically significant; ***p < 0.001, **p < 0.01.Back to article page