Figure 6

Immunofluorescent (IF) staining for inflammatory cells (macrophages) at 24 h and 72 after ischemia-reperfusion (IR) procedure (n = 6 at each time for each group). A to D) and F to I) IF microscopic findings (200 x) showing the number (No.) of CD68+ cell infiltration (white arrows) in kidney parenchyma among four groups at 24 h (A to D) and 72 h (F to I) after IR procedure, respectively. E) The analytical results of CD68+ cells in kidney parenchyma at 24 h after IR procedure. * vs. other groups with different symbols (*, †, ‡, §), p < 0.0001. J) The analytical result of CD68+ cells in renal parenchyma at 72 h after IR procedure. * vs. other groups with different symbols (*, †, ‡, §), p < 0.0001. Scale bars in right lower corner represent 50 μm. All statistical analyses were with one-way ANOVA followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡, §) indicate significance at the 0.05 level. SC = sham control; IR = ischemia-reperfusion; Sita = sitagliptin; Ex-4 = extendin-4.