Figure 2

Serum levels of blood urea nitrogen (BUN), creatinine and glucagon-like peptide-1 (GLP-1), urine amount and ratio of urine protein to creatinine after ischemia-reperfusion (IR) procedure (n = 6 at each time for each group). A) Baseline serum BUN level; p value >0.5 among the four groups. B) Serum level of BUN at 24 h after IR procedure; * vs. other groups with different symbols, p < 0.001. C) Serum level of BUN at 72 h after IR procedure; * vs. other groups with different symbols, p < 0.001. D) Baseline serum creatinine level; p value >0.5 among the four groups. E) Serum level of creatinine at 24 h after IR procedure; * vs. other groups with different symbols, p < 0.001. F) Serum level of creatinine at 72 h after IR procedure; * vs. other groups with different symbols, p < 0.001. G) Baseline urine amount; p value >0.5 among the four groups. H) Urine amount at 72 h; * vs. other groups with different symbols, p < 0.001. I) Baseline ratio of urine protein to creatinine; p value >0.5 among the four groups. J) Ratio of urine protein to creatinine at 27 after IR procedure; * vs. other groups with different symbols, p < 0.005. K) Baseline serum concentration of GLP-1; p value >0.5 among the four groups. L) Serum concentration of GLP-1 at 24 h after IR procedure; * vs. other groups with different symbols, p < 0.005. M) Serum concentration of GLP-1 at 72 h after IR procedure; * vs. other groups with different symbols, p < 0.001. Statistical analysis using one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 6). Symbols (*, †, ‡) indicate significant difference (< 0.05). SC = sham control; IR = ischemia-reperfusion; Sita = sitagliptin; Ex-4 = extendin-4.