Table 2 Culture time, population doublings (pds), and growth rate of chondrocytic subpopulation after MACS separation
From: Maintenance of “stem cell” features of cartilage cell sub-populations during in vitro propagation
W5C5 sorting | |||||||||
|---|---|---|---|---|---|---|---|---|---|
Cell type / | Mean | no. of | Mean | Population doublings (pds) /day | |||||
medium | culture time | passages | pds | Unsorted | W5C5+ | W5C5- | |||
[d] | Mean | sd | Mean | sd | Mean | sd | |||
chondrocytes | 63.50 | 4 - 6 | 9.47 | 0.16 | 0.04 | 0.15 | 0.04 | 0.16 | 0.04 |
n = 4 | |||||||||
chondrocytes | 24.50 | 4 | 11.53 | 0.51 | 0.07 | 0.49 | 0.05 | 0.48 | 0.04 |
MSCGM; n = 2 | |||||||||
disc cells | 25.67 | 4 | 11.44 | 0.42 | 0.13 | 0.47 | 0.12 | 0.47 | 0.14 |
n = 3 | |||||||||
W8B2 sorting | |||||||||
Cell type / | Mean | no. of | Mean | population doublings (pds) /day | |||||
medium | culture time | passages | pds | Unsorted | W8B2+ | W8B2- | |||
[d] | mean | sd | mean | sd | mean | sd | |||
chondrocytes | 70.00 | 4 - 6 | 10.57 | 0.15 | 0.01 | 0.14 | 0.01 | 0.15 | 0.01 |
n = 3 | |||||||||