Co-staining of TGase-4, FAK and paxillin in prostate cancer cells. A: Immunofluorescence staining of FAK in PC-3 (A1) and CA-HPV-10 (A2) cells. Wild type and the transfection control PC-3 cells showed little staining of focal adhesion complex. However, TGase-4 transfected (PC3 TGase4exp) spread well over the matrix and displayed a characteristic staining of focal adhesion complex using the anti-FAK antibody. B: PC3-TGase4exp cells: Localisation FAK (B1 left), paxillin (B1 middle), integrin-β1 (B1 right) and TGase-4 (B2), and co-localisation of FAK/paxillin/integrin with TGase-4 (B3). All images were from PC-3 cells transfected with TGase-4 (PC-3 TGase4exp). FAK/paxillin/integrin demonstrated a clear co-localisation with TGase-4 in the cells.