Figure 4

TGase-4 protein domains and cell-matrix adhesion. PC-3 cells were transfected with a series of plasmids that coded mutant TGase-4 with either TGase-N domain deleted, N- and core- domains deleted, TGase-C domain deleted, C- and core-domains deleted, the short core domain or the large core domain (A and B). Cell adhesion after transfection was determined using ECIS in the absence or presence of PF-573228 (C and D). PC-3 transfected with core domain containing domains (B/C/D/E) showed increased matrix adhesion, similar to that seen in cells transfected with the full length TGase-4. Cells transfected with plasmids in which core domains (F/G) were deleted did not differ from control cells. Increased adhesion mediated by TGase-4 full length, and core domains was inhibited by PF-573228. * p < 0.05 vs no-PF573228 control, ** p < 0.05 vs transfection control.