Effects of TGase-4 expression and cell-matrix adhesion of prostate cancer cells. A and B: Western blotting analysis of protein expression of TGase-4 after transfections for PC-3 (A) and CA HPV-10 (B) cells. Bottom panel is the TGase-4/GAPDH ratio. C: Over-expression of TGase-4 in PC-3 cells signficantly increased matrix adhesion. *p < 0.05 vs no HGF, ** p < 0.05 vs control cells. D: Effects of TGase-4 knockdown on the in vivo invasiveness of CA-HPV-10 cells. Reduction of TGase-4 significantly reduced the invasiveness of the prostate cancer cells. *p < 0.05 vs no HGF, ** p < 0.05 vs control cells. E and F: ECIS based analysis of matrix adhesion of PC-3 (E) and CA-HPV-10 (F) cells. Over-expression of TGase-4 in PC-3 cells markedly increased the pace of matrix adhesion compared with the control cells (E). In contrast, knocking down TGase-4 marked reduced the adhesiveness.