Figure 3

Validation of results for mRNA and serum protein levels of IGFBP1. (A) qRT-PCR was used to validate hepatic Igfbp1 gene expression. The open bars were the control mice; black bars were for the alcohol-treated animals. (B) Serum levels of IGFBP1 protein measured by ELISA. Increases in the protein levels generally mimicked changes seen for microarray and qRT-PCT results. Data were for samples from individual mice, six animals in each group. For statistical significance, “n.s.” stands for no significance; “**” indicates a significant difference with a p-value less than 0.01.