Figure 2

MiR-370 enhanced HHT-induced apoptosis dependent in part on FoxM1 in K562 cells. (A) The mRNA expression of FoxM1 was regulated by miR-370 mimics or inhibitor at the transcriptional level. Quantitative RT-PCR (qRT-PCR) analysis of miR-370 and FoxM1 and the loading control U6 snRNA. The time for transfection of miR-370 and incubation of 0.015 μM HHT is 72 h. Data are mean ± SEM of 3 independent experiments. (B) Western blot analysis of FoxM1 protein level after treatment with miR-370 mimics and inhibitor. β-actin was a loading control. (C) after transfection with miR-370 mimics with or without HHT. (D) Quantitative flow cytometry of the apoptosis of K562 cells. Cells were harvested 72 h after transfection of miR-370 mimics or FoxM1 overexpression plasmid. The results are from 3 independent experiments. (E) Cells were harvested 72 h after transfection of miR-370 inhibitor or FoxM1 special siRNA (5 μM). Quantitative flow cytometry of the apoptosis of K562 cells. The results are from 3 independent experiments. *P < 0.05, **P < 0.01.