Figure 3
From: Preserved endothelial function in human obesity in the absence of insulin resistance
Detection of superoxide generation in vessel segments from morbid obese subjects without insulin resistance (NIR-MO) and with insulin resistance (IR- MO) and the effect of mitochondria antioxidant, mito -TEMPO and the anti- TNF-α, infliximab on superoxide generation in IR-MO microarteries. Representative photomicrographs (A) and quantitative analysis (B) of the staining (red signal) with the superoxide-detecting fluorescent probe, dihydroethidium (DHE), in mesenteric microvessels derived from control, NIR-MO and IR-MO subjects treated or not with the superoxide scavenger targeted to the mitochondria, mito-TEMPO (5 μmol/l), and the anti-TNF-α, infliximab (100 μmol/l) on superoxide generation in IR-MO vessels. Original magnification is (20X). *p< 0.001 vs control subjects; p < 0.01 vs NIR-MO subjects; † p< 0.05 vs IR-MO subjects respectively. Each column represents the mean±SEM of 4 to 6 experiments. Panel (C) shows the correlation between the percentages of DHE positive nuclei and pD2 values for BK.