Figure 3

RECK expression is tumor-specific. The average staining intensity for RECK decreased to 13% in tumor tissue compared to normal tissue, whereas EMMPRIN staining remained unchanged (A). Mean RECK staining intensities for the tumor subtypes ± standard error of the mean are shown in (B). In comparison to their normal counterparts, RECK values increased gradually from 6% in clear cell carcinomas (CC) over 38% in papillary carcinomas (Pap.) and 45% in chromophobe carcinomas (Chrom.) to 96% in oncocytomas (Oncocyt.). P values are given above the pairs of columns (A, B). RECK and EMMPRIN antibodies were checked in Western blots (C). RECK bands at 125 kDa and 90 kDa were detected in protein lysates of 8 pairs of tumor and adjacent normal renal tissue (control: beta-actin). These samples were also used to show the panel of EMMPRIN bands, in our blot migrating between 35 and 74 kDa (control: beta-actin).