Table 1 The sequence of primers used in this study

Real-time PCR Primers:

Ad-Endo (290 bp)

Forward

5′-TGACTGCCTCCAAGTAGGCTAGA-3′

Within the Endostatin fragment

Reverse

5′-CCCAGATCCGCGTTAAGA-3′

At the junction of the poly_A signal and the Ad backbone

dl 1520 (264 bp)

Forward

5′-TGTTTCCAGAACTGAGACGCAT-3′

E1B region (Ad2/2261-2281 nt)

Reverse

5′ –TCTCATCGTACCTCAGCACCTT-3′

E1B region (Ad2 3330–3351 nt)

Total Ad (237 bp)

Forward

5′-TCGAAGCCGTTGATGTTGTG-3′

E2B (Ad2/7519-7538 nt or Ad5/7529-7548 nt)

Reverse

5′- GGCCATAGGTCGCCAGTTTA-3′

E2B (Ad2/7519-7538 nt or Ad5/7529-7548 nt)

β-Actin (266 bp)

Forward

5′-CCTTTCCTTCCCAGGGCGTGAT-3′

At the intron 2-exon 3 junction

Reverse

5′-CGGGCCACTCACCTGGGTCAT-3′

Within the exon 3

p53 (298 bp)

Forward

5′-GTGGTGGTGCCCTATGAG-3′

Reverse

5′-AGGAGCTGGTGTTGTTGG-3′

p14^ARF (282 bp)

Forward

5′-CGCGAGTGAGGGTTTTCGT-3′

Reverse

5′-CAGCACCACCAGCGTGTCC-3′

GAPDH (258 bp)

Forward

5′-AGAAGGCTGGGGCTCATTTG -3′

Reverse

5′-AGGGGCCATCCACAGTCTTC-3′

Cloning primers (for cloning into HindIII/EcoRI site of pcDNA3.1(+)):

Ad E1A

Forward

5′-cccaagctt CGGGACTGAAAATGAGAC-3′

E1A gene (Ad2 548 nt – 1554 nt) (942 bp)

Reverse

5′- ccggaattc CAGGTTTACACCTTATGGC-3′

Ad E1B19k

Forward

5′-cccaagctt ATCTTGGTTACATCTGACCTC-3′

E1B19 kDa (Ad2 1690 nt – 2255 nt) (566 bp)

Reverse

5′-ccggaattc AGCCACCTGTACAACATTC-3′

Ad E1A + E1B19k

Forward

5′-cccaagctt CGGGACTGAAAATGAGAC-3′

E1A+E1B19 kDa (Ad2 548 nt – 2255 nt) (1708 bp)

Reverse

5′-ccggaattc AGCCACCTGTACAACATTC-3′