Figure 2

dl 1520 rescued the replication of Ad-Endo in GC cells by supplying the E1A and E1B19k gene products. The replication of Ad-Endo is presented as the increase in the Ad-Endo DNA copy number, which was determined by real-time PCR. The results are shown here as the fold change in the Ad-Endo DNA copy number at the indicated time points relative to that at 0 hours post-infection. A) Ad-Endo DNA copies in GC cells at different time points after infection with 10 MOIs of Ad-Endo alone or in combination with 10 MOIs of dl 1520 (two-way ANOVA, *p<0.05, **p<0.01 compared to that at 0 hours post-infection). B) The Ad-Endo DNA copy number in GC cells at 48 hours after infection with 10 MOIs of Ad-Endo alone or in combination with increasing MOIs of dl 1520 (one-way ANOVA, *p<0.05, **p<0.01 compared to that of dl 1520 at 0 MOIs). C) The Ad-Endo DNA copy number in MGc80-3 cells transiently transfected with the E1A or/and E1B19k genes 48 hours after infection with 10 MOIs of Ad-Endo (one-way ANOVA, **p<0.01 compared to the control pCD-EGFP). The ectopic expression of the E1A and E1B19k genes in MGc80-3 cells was analyzed by Western blotting (actin was used as the internal control) (C, Left).