Figure 4From: Detection of serological biomarkers by proximity extension assay for detection of colorectal neoplasias in symptomatic individualsDose–response and technical specificity for candidate biomarkers. Technical specificity is assessed by comparing standard curves prepared from a serial dilution of antigen mixes with (specific) or without (unspecific) the specific antigen present. These different solution mixes are PBS + 0.1% BSA ± antigen (Additional file 1: Table S4). The x-axis shows the antigen concentrations in the different mixes. The y-axis shows Cp-values, which were normalized to the internal control GFP by subtracting each biomarker value from the GFP-value for this sample. As indicated, each assay was tested by three different specific antigen mixes and a single unspecific antigen mix, which indicate the background of the assay.Back to article page