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Table 2 Compiles the results of immunohistochemical evaluation for activated caspase-3 positivity as well as nitrotyrosine positivity for the different organs at the different time-points

From: Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)

Organ/IHC LD prior to perfusion LD after perfusion LD after CIT BD prior to perfusion BD after perfusion BD after CIT
Heart:       
Caspase-3 0 0 2 ± 0.2 *** 0 1 ± 0.2 2 ± 0.1 +++, ###
Nitrotyrosine 0 1 ± 0.3 2 ± 0.2 ***, ## 0 1 ± 0.1 3+++, °°°
Liver:       
Caspase-3 0 0 1 ± 0.2 * 1 ± 0.1 ** 1 ± 0.2 2 ± 0.2 +++, °°°,§§§
Nitrotyrosine 0 0 1 ± 0.2 ***, ## 2 ± 0.6 *** 2 ± 0.3 3 ++, °°, §§§
Kidney:       
Caspase-3 0 0 1 ± 0.1 * 1 ± 0.2 1 ± 0.2 3 ± 0.1 +++, °°°, §§§
Nitrotyrosine 0 0 2 ± 0.2 ***, ### 1 ± 0.2 ** 1 ± 0.2 2 ± 0.1 ++
  1. For quantification of immunohistochemistry three blinded, independent persons analysed the slides in a semi-quantitative manner (negative, slightly positive, positive, strongly positive) and the results were transferred to a score form 0–3. The mean of the observations was used for statistical analysis. BD: Brain death; CIT: cold ischemic time; IHC: Immunohistochemistry; LD: Living donation; * p < 0.05; ** p < 0.01; *** p < 0.001 as compared to LD prior to perfusion. ++ p < 0.01; +++ p < 0.001 as compared to BD prior to perfusion. °° p < 0.01; °°° p < 0.001 as compared to BD after perfusion. ## p < 0.01; ### p < 0.001 as compared to LD after perfusion. § p < 0.01; §§§ p < 0.001 as compared to LD after CIT. CIT was 4 hours for heart tissue, 6 hours for liver tissue as well as 15 hours for kidney tissue.