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Table 2 Compiles the results of immunohistochemical evaluation for activated caspase-3 positivity as well as nitrotyrosine positivity for the different organs at the different time-points

From: Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)

Organ/IHC

LD prior to perfusion

LD after perfusion

LD after CIT

BD prior to perfusion

BD after perfusion

BD after CIT

Heart:

      

Caspase-3

0

0

2 ± 0.2 ***

0

1 ± 0.2

2 ± 0.1 +++, ###

Nitrotyrosine

0

1 ± 0.3

2 ± 0.2 ***, ##

0

1 ± 0.1

3+++, °°°,§

Liver:

      

Caspase-3

0

0

1 ± 0.2 *

1 ± 0.1 **

1 ± 0.2

2 ± 0.2 +++, °°°,§§§

Nitrotyrosine

0

0

1 ± 0.2 ***, ##

2 ± 0.6 ***

2 ± 0.3

3 ++, °°, §§§

Kidney:

      

Caspase-3

0

0

1 ± 0.1 *

1 ± 0.2

1 ± 0.2

3 ± 0.1 +++, °°°, §§§

Nitrotyrosine

0

0

2 ± 0.2 ***, ###

1 ± 0.2 **

1 ± 0.2

2 ± 0.1 ++

  1. For quantification of immunohistochemistry three blinded, independent persons analysed the slides in a semi-quantitative manner (negative, slightly positive, positive, strongly positive) and the results were transferred to a score form 0–3. The mean of the observations was used for statistical analysis. BD: Brain death; CIT: cold ischemic time; IHC: Immunohistochemistry; LD: Living donation; * p < 0.05; ** p < 0.01; *** p < 0.001 as compared to LD prior to perfusion. ++ p < 0.01; +++ p < 0.001 as compared to BD prior to perfusion. °° p < 0.01; °°° p < 0.001 as compared to BD after perfusion. ## p < 0.01; ### p < 0.001 as compared to LD after perfusion. § p < 0.01; §§§ p < 0.001 as compared to LD after CIT. CIT was 4 hours for heart tissue, 6 hours for liver tissue as well as 15 hours for kidney tissue.