Figure 5
Expression of ZAG. Enlarged image of ZAG in 2DE gel with the histogram of the normalized volume (M±SD) (A), and validation of ZAG with mono-dimensional western blot (B), and 2DE western blot (C). With 2DE immunoblotting we found 4 spots corresponding to ZAG (spot 1- spot 4) with pI values ranging from 5.4 (spot 1) to 5.9 (spot 4). The decrease in CFS in respect to control was observed for all spots detected by immunoblotting. (* p< 0.05, ** p< 0.01, *** p< 0.001, ns not significant).