Figure 6
Target validation of DEP in tissue samples of CA and AA. A) Real-time qRT-PCR, B) A representative of Western blotting analysis. A) Real-time qRT-PCR detected the relative mRNA expression levels of transferrin (TF), apolipoprotein A1 (APOA1), hepatocyte nuclear factor4α (HNF4α) and filamin A (FLNA). 18S rRNA was used as the normalization standard. Compared to CA, AA tissues had an obvious up-regulation of TF in CIR and HCC samples (p < 0.05 and 0.001, respectively) and up-regulation of APOA1, and HNF4α in HCC samples (p < 0.001; p < 0.05, respectively). Bars = means ± SD. There was no significant difference in the expression of FLNA between the two groups. B) A representative of immunoblot analysis result of (I) TF and APOA1, (II) HNF4α in tissue samples of AA and CA. GAPDH was used as a loading control. Compared to CA, AA tissue samples (CIR, HCC) show an obvious increase in protein levels of TF and APOA1, but decreased protein levels of HNF4α.