Figure 3
From: Downregulation of KPNA2 in non-small-cell lung cancer is associated with Oct4 expression
Loss of Oct4 or KPNA2 expression inhibits proliferation in tumor cells. Expression levels of Oct4 and KPNA2 were analyzed by western blotting in a panel of lung cancer cell lines (A). Each bar represents the mean ± SD of three independent experiments. A549 and SPC cells were treated with Oct4- or KPNA2-specific siRNAs for 72 h. Western blot of Oct4 and KPNA2 depletion efficiency in A549 and SPC cell lines (B). Cell growth rate was determined by the CCK-8 assay, as described in Section 2. The data are mean ± SD of three independent experiments (C). (Upper panel) A549 and SPC cells transduced with siRNAs for control, Oct4, or KPNA2 were subjected to a colony formation assay. (Lower panel) Histogram quantification (D). Error bars indicate ± SD.*P < 0.05, **P < 0.01.
