Expression of Oct4 and KPNA2. Immunohistochemical staining for Oct4 and KPNA2 expression in cancerous tissues and their corresponding paracancerous tissues. Brown grains represent a positive signal. The positive expression site of Oct4 and KPNA2 was mainly in the nucleus of tumor cells. Oct4 negative expression in paracancerous tissue (Para) (A); Oct4 positive expression in lung adenocarcinoma (Ad) (B) and lung squamous cell carcinoma (SCC) (C); KPNA2 negative expression in Para (D); KPNA2 positive expression in lung Ad (E) and lung SCC (F). Western blot analysis of the expression of Oct4 and KPNA2 in lung Ad, lung SCC, and Para (G). Each bar represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01. Immunohistochemical staining for Oct4 (H) and KPNA2 (I) in a case of lung SCC. Immunohistochemical staining for Oct4 (J) and KPNA2 (K) in a case of lung Ad. Original magnification, 400 × .